Endoreduplication is not involved in bundle-sheath formation in the C4 species Cleome gynandra

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Endoreduplication is not involved in bundle-sheath formation in the C4 species Cleome gynandra

There is currently significant interest in engineering the two-celled C4 photosynthesis pathway into crops such as rice in order to increase yield. This will require alterations to the biochemistry of photosynthesis in both mesophyll (M) and bundle-sheath (BS) cells, but also alterations to leaf anatomy. For example, the BS of C4 species is enlarged compared with that in C3 species. Because cel...

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The dicotyledonous NAD malic enzyme C4 plant Cleome gynandra displays age-dependent plasticity of C4 decarboxylation biochemistry.

The C(4) photosynthetic pathway enriches carbon dioxide in the vicinity of Rubisco, thereby enabling plants to assimilate carbon more efficiently. Three canonical subtypes of C(4) exist, named after their main decarboxylating enzymes: NAD-dependent malic enzyme type, NADP-dependent malic enzyme type and phosphoenolpyruvate carboxykinase type. Cleome gynandra is known to perform NAD-ME type C(...

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Elevated expression of PGR5 and NDH-H in bundle sheath chloroplasts in C4 flaveria species.

Cyclic electron transport around PSI has been proposed to supply the additional ATP required for C(4) photosynthesis. To investigate the nature of cyclic electron pathways involved in C(4) photosynthesis, we analyzed tissue-specific expression of PGR5 (PROTON GRADIENT REGULATION 5), which is involved in the antimycin A-sensitive pathway, and NDH-H, a subunit of the plastidial NAD(P)H dehydrogen...

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Agrobacterium tumefaciens-mediated transformation of Cleome gynandra L., a C4 dicotyledon that is closely related to Arabidopsis thaliana

In leaves of most C(4) plants, the biochemistry of photosynthesis is partitioned between mesophyll and bundle sheath cells. In addition, their cell biology and development also differs from that in C(3) plants. We have a poor understanding of the mechanisms that generate the cell-specific accumulation of proteins used in the C(4) pathway, and there are few genes that have been shown to be impor...

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Phosphoenolpyruvate carboxykinase is involved in the decarboxylation of aspartate in the bundle sheath of maize

We recently showed that maize (Zea mays L.) leaves contain appreciable amounts of phosphoenolpyruvate carboxykinase (PEPCK; R.P. Walker, R.M. Acheson, L.I. Tecsi, R.C. Leegood [1997] Aust J Plant Physiol 24: 459-468). In the present study, we investigated the role of PEPCK in C4 photosynthesis in maize. PEPCK activity and protein were enriched in extracts from bundle-sheath (BS) strands compare...

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ژورنال

عنوان ژورنال: Journal of Experimental Botany

سال: 2013

ISSN: 1460-2431,0022-0957

DOI: 10.1093/jxb/ert350